Abstract

Antibiotic resistance has emerged as one of the greatest global health challenges to be addressed in the 21st
century.The reintroduction of previously used antibiotics active against extensively drug-resistant (XDR)
bacteria represents a new alternative for the control of antimicrobial resistance. A total of 100 extensively
drug resistant Gram negative bacilli isolated from Clinical Microbiology laboratory.Acinetobacter was
found to be the most common and highly resistant organism in this study (54%), which was isolated mostly
from respiratory and pus samples.Other extensively drug resistant Gram negative bacilli isolated from
Clinical samples includes Klebsiella (33%) and Pseudomonas(13%).From the 100 Extensively drug
resistant gram negative bacteria, the most common respiratory pathogen Acinetobacter shows 78%
sensitivity towards Minocycline followed by Klebsiella (21%). Pseudomonas shows complete resistance
towards this drug.The old antibiotic Fosfomycin was active against all three species (Acinetobacter 94%,
Klebsiella 100% and Pseudomonas 77%).Chloramphenicol shows less sensitivity towards Klebsiella (33%)
and Acinetobacter(22%) and pseudomonas(16%).97% isolates were sensitive to polymyxins\colistin.